01781nas a2200169   4500008004100000022001400041245008200055210006900137260001600206490000900222520124900231100001801480700001701498700002101515700002001536856005501556       2011                        ENG d  a0277-786X00aInstrument-free nucleic acid amplification assays for global health settings.0 aInstrumentfree nucleic acid amplification assays for global heal  c2011 May 160 v80293 a<p>Many infectious diseases that affect global health are most accurately diagnosed through nucleic acid amplification and detection. However, existing nucleic acid amplification tests are too expensive and complex for most low-resource settings. The small numbers of centralized laboratories that exist in developing countries tend to be in urban areas and primarily cater to the affluent. In contrast, rural area health care facilities commonly have only basic equipment and health workers have limited training and little ability to maintain equipment and handle reagents.(1) Reliable electric power is a common infrastructure shortfall. In this paper, we discuss a practical approach to the design and development of non-instrumented molecular diagnostic tests that exploit the benefits of isothermal amplification strategies. We identify modular instrument-free technologies for sample collection, sample preparation, amplification, heating, and detection. By appropriately selecting and integrating these instrument-free modules, we envision development of an easy to use, infrastructure independent diagnostic test that will enable increased use of highly accurate molecular diagnostics at the point of care in low-resource settings.</p>1 aLaBarre, Paul1 aBoyle, David1 aHawkins, Kenneth1 aWeigl, Bernhard  uhttp://microfluidicsciences.com/drupal/?q=node/210