01142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2014                        eng d00aUnited {States} {Patent}: 8697009 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8697009 Microfluidic devices for fluid mani  capr3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=3&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2013                        eng d00aUnited {States} {Patent}: 8557198 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8557198 Microfluidic devices for fluid mani  coct3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=4&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201142nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023000742       2012                        eng d00aUnited {States} {Patent}: 8318109 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 8318109 Microfluidic devices for fluid mani  cnov3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=6&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202044nas a2200169   4500008004100000245012100041210006900162260000800231520126200239100002501501700002701526700002101553700002401574700002201598700002401620856023001644       2010                        eng d00aUnited {States} {Patent}: 7648835 - {System} and method for heating, cooling and heat cycling on microfluidic device0 aUnited States Patent 7648835 System and method for heating cooli  cjan3 aAn integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.1 aBreidford, Wayne, L.1 aLancaster, Christy, A.1 aHayenga, Jon, W.1 aBardell, Ronald, L.1 aTonn, Jeffrey, F.1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=9&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2202046nas a2200169   4500008004100000245012100041210006900162260000800231520126200239100002101501700002701522700002601549700002401575700002201599700002401621856023101645       2009                        eng d00aUnited {States} {Patent}: 7544506 - {System} and method for heating, cooling and heat cycling on microfluidic device0 aUnited States Patent 7544506 System and method for heating cooli  cjun3 aAn integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card.1 aBreidford, Wayne1 aLancaster, Christy, A.1 aHayenga, Jon, Wallace1 aBardell, Ronald, L.1 aTonn, Jeffrey, F.1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=11&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201618nas a2200169   4500008004100000245018300041210006900224260000800293520077700301100002401078700001901102700002401121700002401145700002701169700002101196856023101217       2008                        eng d00aUnited {States} {Patent}: 7416892 - {Method} and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing0 aUnited States Patent 7416892 Method and system for microfluidic   caug3 aA microfluidic system for isolation and amplification of DNA or RNA from aqueous solutions and detection of the DNA or RNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA or RNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA or RNA are transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays.1 aBattrell, Frederick1 aShen, Mingchao1 aWeigl, Bernhard, H.1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBreidford, Wayne  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=13&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%2201143nas a2200169   4500008004100000245009900041210006900140260000800209520038500217100002200602700001900624700002400643700002700667700002400694700002400718856023100742       2008                        eng d00aUnited {States} {Patent}: 7419638 - {Microfluidic} devices for fluid manipulation and analysis0 aUnited States Patent 7419638 Microfluidic devices for fluid mani  csep3 aThe present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.1 aSaltsman, Patrick1 aShen, Mingchao1 aHoukal, Jeffrey, M.1 aLancaster, Christy, A.1 aBattrell, Frederick1 aWeigl, Bernhard, H.  uhttp://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=12&f=G&l=50&co1=AND&d=PTXT&s1=%22Weigl%3B+Bernhard%22.INNM.&OS=IN/%22Weigl;+Bernhard%22&RS=IN/%22Weigl;+Bernhard%22