01932nas a2200313   4500008004100000022001400041245011800055210006900173260001600242300001100258490000800269520098000277653001201257653002101269653001401290653001001304653002901314653000901343653001801352653002501370653002301395653002401418100002601442700001801468700002501486700002601511700002701537856005401564       2002                        eng d  a0378-517300aLiposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating.0 aLiposome clearance in mice the effect of a separate and combined  c2002 Jun 20  a95-1020 v2403 a<p>The purpose of our work was to compare the biodistribution of liposomes with different surface properties. Phosphatidylcholine (PC)/cholesterol (Chol) liposomes were prepared containing 6% mol of a charged lipid (stearylamine, SA; phosphatidic acid, PA; or phosphatidyl serine, PS) and/or polyethylene glycol (PEG)-PE of different MW (750 and 5000). zeta-Potentials and liposome clearance in mice were investigated. In vitro, the attachment of PEG in a similar fashion neutralizes the effect of any charged component. In vivo, the chemical nature of a charged lipid becomes important. Both short PEG750 and longer PEG5000 inhibit the clearance of positively charged SA-liposomes, while only longer PEG5000 inhibits the clearance of negatively charged PA-liposomes and none of the PEGs inhibit the clearance of negatively charged PS-liposomes. The opsonins with different molecular size may be involved in the clearance of liposomes containing different charged lipids.</p>10aAnimals10aElectrochemistry10aLiposomes10aLiver10aMetabolic Clearance Rate10aMice10aParticle Size10aPolyethylene Glycols10aSurface Properties10aTissue Distribution1 aLevchenko, Tatiana, S1 aRammohan, Ram1 aLukyanov, Anatoly, N1 aWhiteman, Kathleen, R1 aTorchilin, Vladimir, P  uhttp://microfluidicsciences.com/drupal/?q=node/28