@article {28,
	title = {Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating.},
	journal = {Int J Pharm},
	volume = {240},
	year = {2002},
	month = {2002 Jun 20},
	pages = {95-102},
	abstract = {<p>The purpose of our work was to compare the biodistribution of liposomes with different surface properties. Phosphatidylcholine (PC)/cholesterol (Chol) liposomes were prepared containing 6\% mol of a charged lipid (stearylamine, SA; phosphatidic acid, PA; or phosphatidyl serine, PS) and/or polyethylene glycol (PEG)-PE of different MW (750 and 5000). zeta-Potentials and liposome clearance in mice were investigated. In vitro, the attachment of PEG in a similar fashion neutralizes the effect of any charged component. In vivo, the chemical nature of a charged lipid becomes important. Both short PEG750 and longer PEG5000 inhibit the clearance of positively charged SA-liposomes, while only longer PEG5000 inhibits the clearance of negatively charged PA-liposomes and none of the PEGs inhibit the clearance of negatively charged PS-liposomes. The opsonins with different molecular size may be involved in the clearance of liposomes containing different charged lipids.</p>},
	keywords = {Animals, Electrochemistry, Liposomes, Liver, Metabolic Clearance Rate, Mice, Particle Size, Polyethylene Glycols, Surface Properties, Tissue Distribution},
	issn = {0378-5173},
	author = {Levchenko, Tatiana S and Rammohan, Ram and Lukyanov, Anatoly N and Whiteman, Kathleen R and Torchilin, Vladimir P}
}
