02026nas a2200397   4500008004100000022001400041245015900055210006900214260001600283300001100299490000800310520079300318653001201111653002601123653003601149653002101185653002201206653001901228653001901247653001101266653001101277653000901288653001301297653002901310653001801339653003001357653002501387653002601412653001601438100001801454700002401472700002501496700002601521700002701547856005401574       2004                        eng d  a0378-517300aPreparation and in vitro synergistic anticancer effect of vitamin K3 and 1,8-diazabicyclo[5,4,0]undec-7-ene in poly(ethylene glycol)-diacyllipid micelles.0 aPreparation and in vitro synergistic anticancer effect of vitami  c2004 Mar 19  a129-350 v2723 a<p>Polymeric micelles consisting of poly(ethylene glycol)-distearoyl phosphoethanolamine conjugates (PEG-DSPE) loaded with Vitamin K3 (VK3) to 0.2 mg of drug/mg of carrier and with 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU) to 0.06 mg of drug/mg of carrier were prepared. These micelles were stable for as long as 6 months during storage at 4 degrees C and did not change their size or release the incorporated drugs. Co-encapsulation of VK3 and DBU into PEG-DSPE micelles resulted in synergistic anticancer effects against both murine and human cancer cells in vitro. The synergism may be explained by the fact that the presence of DBU promotes the escape of drug-loaded micelles from the endosomes of cancer cells directly into the cytoplasm as demonstrated by fluorescent microscopy.</p>10aAnimals10aAntineoplastic Agents10aBicyclo Compounds, Heterocyclic10aCell Line, Tumor10aDrug Combinations10aDrug Stability10aDrug Synergism10aFemale10aHumans10aMice10aMicelles10aMicroscopy, Fluorescence10aParticle Size10aPhosphatidylethanolamines10aPolyethylene Glycols10aTumor Cells, Cultured10aVitamin K 31 aWang, Junping1 aMongayt, Dmitriy, A1 aLukyanov, Anatoly, N1 aLevchenko, Tatiana, S1 aTorchilin, Vladimir, P  uhttp://microfluidicsciences.com/drupal/?q=node/1901932nas a2200313   4500008004100000022001400041245011800055210006900173260001600242300001100258490000800269520098000277653001201257653002101269653001401290653001001304653002901314653000901343653001801352653002501370653002301395653002401418100002601442700001801468700002501486700002601511700002701537856005401564       2002                        eng d  a0378-517300aLiposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating.0 aLiposome clearance in mice the effect of a separate and combined  c2002 Jun 20  a95-1020 v2403 a<p>The purpose of our work was to compare the biodistribution of liposomes with different surface properties. Phosphatidylcholine (PC)/cholesterol (Chol) liposomes were prepared containing 6% mol of a charged lipid (stearylamine, SA; phosphatidic acid, PA; or phosphatidyl serine, PS) and/or polyethylene glycol (PEG)-PE of different MW (750 and 5000). zeta-Potentials and liposome clearance in mice were investigated. In vitro, the attachment of PEG in a similar fashion neutralizes the effect of any charged component. In vivo, the chemical nature of a charged lipid becomes important. Both short PEG750 and longer PEG5000 inhibit the clearance of positively charged SA-liposomes, while only longer PEG5000 inhibits the clearance of negatively charged PA-liposomes and none of the PEGs inhibit the clearance of negatively charged PS-liposomes. The opsonins with different molecular size may be involved in the clearance of liposomes containing different charged lipids.</p>10aAnimals10aElectrochemistry10aLiposomes10aLiver10aMetabolic Clearance Rate10aMice10aParticle Size10aPolyethylene Glycols10aSurface Properties10aTissue Distribution1 aLevchenko, Tatiana, S1 aRammohan, Ram1 aLukyanov, Anatoly, N1 aWhiteman, Kathleen, R1 aTorchilin, Vladimir, P  uhttp://microfluidicsciences.com/drupal/?q=node/28