02026nas a2200397   4500008004100000022001400041245015900055210006900214260001600283300001100299490000800310520079300318653001201111653002601123653003601149653002101185653002201206653001901228653001901247653001101266653001101277653000901288653001301297653002901310653001801339653003001357653002501387653002601412653001601438100001801454700002401472700002501496700002601521700002701547856005401574       2004                        eng d  a0378-517300aPreparation and in vitro synergistic anticancer effect of vitamin K3 and 1,8-diazabicyclo[5,4,0]undec-7-ene in poly(ethylene glycol)-diacyllipid micelles.0 aPreparation and in vitro synergistic anticancer effect of vitami  c2004 Mar 19  a129-350 v2723 a<p>Polymeric micelles consisting of poly(ethylene glycol)-distearoyl phosphoethanolamine conjugates (PEG-DSPE) loaded with Vitamin K3 (VK3) to 0.2 mg of drug/mg of carrier and with 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU) to 0.06 mg of drug/mg of carrier were prepared. These micelles were stable for as long as 6 months during storage at 4 degrees C and did not change their size or release the incorporated drugs. Co-encapsulation of VK3 and DBU into PEG-DSPE micelles resulted in synergistic anticancer effects against both murine and human cancer cells in vitro. The synergism may be explained by the fact that the presence of DBU promotes the escape of drug-loaded micelles from the endosomes of cancer cells directly into the cytoplasm as demonstrated by fluorescent microscopy.</p>10aAnimals10aAntineoplastic Agents10aBicyclo Compounds, Heterocyclic10aCell Line, Tumor10aDrug Combinations10aDrug Stability10aDrug Synergism10aFemale10aHumans10aMice10aMicelles10aMicroscopy, Fluorescence10aParticle Size10aPhosphatidylethanolamines10aPolyethylene Glycols10aTumor Cells, Cultured10aVitamin K 31 aWang, Junping1 aMongayt, Dmitriy, A1 aLukyanov, Anatoly, N1 aLevchenko, Tatiana, S1 aTorchilin, Vladimir, P  uhttp://microfluidicsciences.com/drupal/?q=node/19