@article {16,
	title = {Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody.},
	journal = {J Control Release},
	volume = {100},
	year = {2004},
	month = {2004 Nov 5},
	pages = {135-44},
	abstract = {<p>Commercially available doxorubicin-loaded long-circulating liposomes (Doxil, Alza Pharmaceuticals) were modified with the monoclonal nucleosome (NS)-specific 2C5 antibody (mAb 2C5) that recognizes a broad variety of tumors via the tumor cell surface-bound NSs. For incorporation into liposomes, mAb 2C5 was modified with poly(ethylene glycol)-phosphatidyl ethanolamine conjugate (PEG-PE) with the free PEG terminus activated with the p-nitrophenylcarbonyl group (pNP-PEG-PE). Derivatives of mAb 2C5 containing a variable number of PEG-PE residues (10-32) per protein molecule were prepared with a reasonably good preservation of the antibody specific activity even at the highest degree of modification. PEG-PE-modified antibody quantitatively incorporated into the liposomal membrane of doxorubicin-loaded liposomes with a loss of not more than 20\% of the encapsulated doxorubicin. 2C5-targeted Doxil liposomes acquired the ability to recognize NSs and specifically bind to various tumor cells. Doxorubicin-loaded long-circulating liposomes modified with the mAb 2C5 kill various tumor cells in vitro with the efficiency higher than non-targeted doxorubicin-loaded liposomes.</p>},
	keywords = {Animals, Antibodies, Monoclonal, Antibodies, Neoplasm, Cell Line, Tumor, Doxorubicin, Humans, Liposomes, Mice, Neoplasms, Nucleosomes},
	issn = {0168-3659},
	doi = {10.1016/j.jconrel.2004.08.007},
	author = {Lukyanov, Anatoly N and Elbayoumi, Tamer A and Chakilam, Ananthsrinivas R and Torchilin, Vladimir P}
}
